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Received 10 October 2005; Revised 23 November 2005; Accepted 3 January 2006; Published online 23 February 2006.Top of pageAbstractWe compared antisense phosphorothioate oligonucleotides (PS ODN) that target BCL 2 such as Genasense (G3139 PS), with other PS ODN or phosphodiester ODN (PO ODN) in their relative capacity to induce apoptosis of chronic lymphocytic leukemia (CLL) B cells in vitro. Surprisingly, we found that thymidine containing PS ODN, but not PO ODN, induced activation and apoptosis of CLL cells independent of BCL 2 antisense sequence or CpG motifs. All tested thimidine containing PS ODN, irrespective of their primary sequences, reduced the expression of Bcl 2 protein and increased the levels of the proapoptotic molecules p53, Bid, Bax in CLL cells.
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